产品货号：
GS4459
中文名称：
重组猴GM-CSF
英文名称：
Recombinant Rhesus Macaque Granulocyte-Macrophage Colony Stimulating Factor
产品规格：
2μg
发货周期：
1～3天
产品价格：
询价
General Description:
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells,B cells,macrophages,mast cells,endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation.It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages.GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate.Additionally,it can stimulate the proliferation of a number of tumor cell lines,including osteogenic sarcoma,carcinoma and adenocarcinoma cell lines.It is reported that GM-CSF has no biological effects across species.
Storage:
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage:
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight:
Approximately 14.4kDa,a single non-glycosylated polypeptide chain containing 127 amino acids.
Purity:
> 98% by SDS-PAGE and HPLC analyses.
Source:
Escherichia coli.
Biological Activity:
Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1ng/mL,corresponding to a specific activity of> 1.0×107 IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a 0.2μm filtered concentrated solution in PBS,pH7.4.
Endotoxin:
Less than 1 EU/μg of rRhGM-CSF as determined by LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.Further dilutions should be made in appropriate buffered solutions.
AA Sequence:
APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTV EVVSEMFDLQ EPSCLQTRLE LYKQGLQGSLTKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFQSFKENL KDFLLVIPFD CWEPVQE
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells,B cells,macrophages,mast cells,endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation.It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages.GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate.Additionally,it can stimulate the proliferation of a number of tumor cell lines,including osteogenic sarcoma,carcinoma and adenocarcinoma cell lines.It is reported that GM-CSF has no biological effects across species.
Storage:
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage:
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight:
Approximately 14.4kDa,a single non-glycosylated polypeptide chain containing 127 amino acids.
Purity:
> 98% by SDS-PAGE and HPLC analyses.
Source:
Escherichia coli.
Biological Activity:
Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1ng/mL,corresponding to a specific activity of> 1.0×107 IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a 0.2μm filtered concentrated solution in PBS,pH7.4.
Endotoxin:
Less than 1 EU/μg of rRhGM-CSF as determined by LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.Further dilutions should be made in appropriate buffered solutions.
AA Sequence:
APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTV EVVSEMFDLQ EPSCLQTRLE LYKQGLQGSLTKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFQSFKENL KDFLLVIPFD CWEPVQE
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